Nelson Higher Education

Higher Education

Basic Clinical Laboratory Techniques, 6th Edition

  • Barbara H. Estridge
  • Anna P. Reynolds
  • ISBN-10: 1111138362
  • ISBN-13: 9781111138363
  • 816 Pages | Paperback
  • Previous Editions: 2008, 2000, 1996
  • COPYRIGHT: 2012 Published
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About the Product

BASIC CLINICAL LABORATORY TECHNIQUES, Sixth Edition teaches prospective laboratory workers and allied health care professionals the basics of clinical laboratory procedures and the theories behind them. Performance-based to maximize hands-on learning, this "work-text" includes step-by-step instruction and worksheets to help users understand laboratory tests and procedures ranging from specimen collection and analysis, to instrumentation and CLIA and OSHA safety protocols. Students and working professionals alike will find BASIC CLINICAL LABORATORY TECHNIQUES an easy-to-understand, reliable resource for developing and refreshing key laboratory skills.


  • Full-color photographs and illustrations depict the laboratory setting today and aid in visual organization.

  • Colorful graphic icons and in-text features center on safety precautions, critical timing, math skills, quality assurance, and other important issues.

  • Suggested web-based activities and numerous review questions throughout the book reinforce readings and laboratory practice worksheets to let users assess their progress.

  • Since safety is always a priority in clinical settings, these procedures are strongly emphasized throughout the lessons, particularly the safety devices, procedures, and technologies that prevent needle-stick injury.

About the Author

Barbara H. Estridge

Barbara H. Estridge is a retired instructor of medical technology and research associate in the College of Science & Mathematics at Auburn University in Auburn, Alabama. Her experience includes working at the hospital bench, teaching clinical laboratory courses, biological and biomedical research, clinical laboratory management, and more.

Anna P. Reynolds

Anna P. Reynolds is a retired instructor of medical technology and a research coordinator at the College of Agriculture at Auburn University. She taught both hospital and university Medical Technology, Medical Laboratory Technician, and Medical Laboratory Assistant programs. Her experience includes clinical and research experience in hematology, chemistry, urinalysis, microbiology, parasitology, and immunology.

Table of Contents

List of Figures and Tables.
How to Use This Book.
The Clinical Laboratory. The Clinical Laboratory Professional. Medical Terminology.
Laboratory Safety: Physical and Chemical Hazards. Laboratory Safety: Biological Hazards. General Laboratory Equipment. The Metric System. Preparing Laboratory Reagents. Quality Assessment. The Microscope. Capillary Puncture. Routine Venipuncture.
Introduction to Hematology. Hemoglobin. Microhematocrit. The Hemacytometer.
Manual RBC and WBC Counts. Platelet Count. Preparing and Staining a Blood Smear.
Normal Blood Cell Morphology. White Blood Cell Differential Count. Principles of Automated Hematology. Abnormalities in Peripheral Blood Cell Morphology. Reticulocyte Count. Erythrocyte Sedimentation Rate.
Principles of Hemostasis. Disorders of Hemostasis. Coagulation Testing Methods.
Prothrombin Time. Activated Partial Thromboplastin Time. D-Dimer.
Introduction to Immunology. Infectious Mononucleosis Tests. Test for Rheumatoid Factors. Urine hCG Tests. Introduction to Immunohematology. ABO Grouping.
Rh Typing.
Introduction to Urinalysis. Urine Collection and Processing. Physical Examination of Urine. Chemical Examination of Urine. Microscopic Examination of Urine Sediment.
Introduction to Clinical Chemistry. Principles of Chemistry Instrumentation.
Chemistry Specimen Collection and Processing. Point-of-Care Testing. Blood Glucose and Hemoglobin A1c. Blood Cholesterol and Triglycerides. Electrolytes. Fecal Occult Blood.
Introduction to Clinical Microbiology. Microbiology Specimen Collection and Processing.
Culture Techniques for Bacteriology. The Gram Stain. Tests for Group A Streptococcus.
Urine Culture and Colony Count. Bacterial Identification and Antibiotic Susceptibility Testing. Tests for Sexually Transmitted Diseases. Infection Prevention in Health-Care Settings. Public Health Issue: Emerging Infectious Diseases. Public Health Issue: Biological Threats.
Introduction to Parasitology. Collecting and Processing of Specimens for Parasite Detection. Microscopic Methods of Detecting Intestinal Parasites. Preparing Smears for Blood Parasites.
Appendix A: Guide to Standard Precautions.
Appendix B: Laboratory Reference Values.
Appendix C: Abbreviations and Acronyms Commonly Used in Clinical Laboratories.
Appendix D: Sources of Information: Health Care Accrediting and Credentialing Agencies, Professional Societies, Governmental Agencies.

New to this edition

  • Expanded and updated lessons include all of the best elements of earlier editions--Objectives, Glossary, Review Questions, and Student Performance Guides—along with significant enhancements to every lesson in order to convey the most current accepted practices.
  • Exciting new technologies and instrumentation such as nephelometry, turbidimetry, and automated laboratory transport systems, as well as bar code use on requisition forms, patient I.D. bracelets, and patient specimens, have all been added.
  • Appealing visual enhancements including new photomicrographs of normal and abnormal blood cells add interest to the study of blood cell morphology, while many new tables and graphs for deeper understanding.
  • All-new specimen lessons include chemistry specimen collection and processing, collection of specimens for microbiology, and the use of transport media.
  • Newly added, helpful case studies and critical thinking questions require readers to apply principles from the lesson to solve problems, preparing them for on-the-job decision making.


All supplements have been updated in coordination with the Main title.
Please see Main title page for new to this edition information.

Instructor Supplements

Instructor's Resources  (ISBN-10: 1111138389 | ISBN-13: 9781111138387)

The Instructor Resources includes PowerPoint® slides that coordinate with the book and assist with classroom presentation; an Instructor's Manual containing an objectives list, an overview for each unit, and lesson plans for each lesson; a test bank created in ExamView™ with over 1,500 questions in true/false, multiple choice, matching, and fill-in-the-blank formats—to make planning your course easier. In addition, all of the Student Performance Guides, Worksheets, and Report Forms from the text can be found in an electronic format on the Instructor Resources CD. NEW TO THIS EDITION: The Instructor Resources CD includes expanded and updated lesson materials from the book that convey the most current accepted practices.

Coursemate Instant Access  (ISBN-10: 1133732313 | ISBN-13: 9781133732310)

In addition to interactive teaching and learning tools, CourseMate includes an interactive eBook. Students can take notes, highlight, search and interact with embedded media specific to their book. Use it as a supplement to the printed text, or as a substitute—the choice is your students' with CourseMate.

Student Supplements

Coursemate Instant Access  (ISBN-10: 1133732313 | ISBN-13: 9781133732310)

In addition to interactive teaching and learning tools, CourseMate includes an interactive eBook. Students can take notes, highlight, search and interact with embedded media specific to their book. Use it as a supplement to the printed text, or as a substitute—the choice is your students' with CourseMate.